https://doi.org/10.1051/epjap:2008176
Glass-based nanofluidic device for biomolecule preconcentration study
1
Laboratoire de Photonique et de Nanostructures, LPN-CNRS, Route de Nozay, 91460 Marcoussis, France
2
Laboratoire pour l'Application de Lasers de Puissance, LALP-CNRS/GIP-GERAILP, 16 bis av. Prieur de la Côte d'Or, 94114 Arcueil Cedex, France
Corresponding author: svarnas@lpn.cnrs.fr
Received:
11
March
2008
Revised:
1
September
2008
Accepted:
12
September
2008
Published online:
24
October
2008
The fabrication of a hybrid micro/nano-fluidic device to study electropreconcentration of biomolecules is presented. A nanoslit surrounded by glass is formed between two access microchannels and an “H” configuration bio-analysis system is developed. The optimized know-how for this biochip fabrication is transferred and the key steps are discussed. Fluorescence spatiotemporal profile in the preconcentration area is recorded and quantitatively analyzed. The reliability of the applied technology for studies of charged biomolecule transport phenomena across nanochannels is proven giving two examples for different preconcentration geometries.
PACS: 47.61.-k – Micro- and nano- scale flow phenomena / 81.16.Rf – Nanoscale pattern formation / 87.85.Qr – Nanotechnologies-design / 87.85.Rs – Nanotechnologies-applications
© EDP Sciences, 2008
